stedycon confocal and sted module Search Results


stedycon confocal and sted module  (abberior instruments)
90
abberior instruments stedycon confocal and sted module
a Log2(tpm+1) data from bulk RNASeq analysis show expression profiles of GLI1 and PTCH1 as mean ± SEM. b , c Immunofluorescence analysis of ciliary proteins in WT and RPGRIP1L-deficient spinal organoids at day 6. b Cilia are labeled by ARL13B and basal bodies by γ-TUBULIN. c Cilia are labeled by IFT81 in green. d , e Cilia length and density measurements in WT and RGPRIP1L -/- organoids at day 6. Data are shown as median with quartiles. Mann-Whitney test was performed for statistical analyzes. f <t>Stimulated-Emission-Depletion</t> <t>(STED)</t> images of WT and RPGRIP1L-deficient cilia at day 6. Cilia are labeled by ARL13B and RPGRIP1L. g Quantification of the ciliary RPGRIP1L amount based on confocal images. Data are shown as median with quartiles. Statistics: unpaired t tests with Welch’s correction ( p < 0.0001). h , i Immunofluorescence of WT and RPGRIP1L-deficient cilia on spinal organoids at day 6. Cilia are labeled by ARL13B and AC3 ( h ) or by ARL13B and INPP5E ( i ). Basal bodies are labeled by γ-TUBULIN. j, k, l Quantifications of ciliary ARL13B ( j ), AC3 ( k ) and INPP5E ( l ) amounts in WT and RPGRIP1L-deficient organoids. Data are shown as median with quartiles. Statistics: unpaired t tests with Welch’s correction ( p < 0.0001). a - l N: number of independent experiments; n: number of different WT or KO clones analyzed per experiment. For a , 2 WT clones and 1 KO clone from each iPSC line ( n = 4 for WT and n = 2 for KO). For d , e , g and j – l , 2 WT clones and 2 KO clones from each line ( n = 4 for each genotype). N = 3 for ( d , e , g , j-l ); N = 1 for a . Scale bars: 300 µm in b; 0.5 µm in c ; 1 µm in h , i .
Stedycon Confocal And Sted Module, supplied by abberior instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stedycon confocal and sted module - by Bioz Stars, 2026-07
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a Log2(tpm+1) data from bulk RNASeq analysis show expression profiles of GLI1 and PTCH1 as mean ± SEM. b , c Immunofluorescence analysis of ciliary proteins in WT and RPGRIP1L-deficient spinal organoids at day 6. b Cilia are labeled by ARL13B and basal bodies by γ-TUBULIN. c Cilia are labeled by IFT81 in green. d , e Cilia length and density measurements in WT and RGPRIP1L -/- organoids at day 6. Data are shown as median with quartiles. Mann-Whitney test was performed for statistical analyzes. f Stimulated-Emission-Depletion (STED) images of WT and RPGRIP1L-deficient cilia at day 6. Cilia are labeled by ARL13B and RPGRIP1L. g Quantification of the ciliary RPGRIP1L amount based on confocal images. Data are shown as median with quartiles. Statistics: unpaired t tests with Welch’s correction ( p < 0.0001). h , i Immunofluorescence of WT and RPGRIP1L-deficient cilia on spinal organoids at day 6. Cilia are labeled by ARL13B and AC3 ( h ) or by ARL13B and INPP5E ( i ). Basal bodies are labeled by γ-TUBULIN. j, k, l Quantifications of ciliary ARL13B ( j ), AC3 ( k ) and INPP5E ( l ) amounts in WT and RPGRIP1L-deficient organoids. Data are shown as median with quartiles. Statistics: unpaired t tests with Welch’s correction ( p < 0.0001). a - l N: number of independent experiments; n: number of different WT or KO clones analyzed per experiment. For a , 2 WT clones and 1 KO clone from each iPSC line ( n = 4 for WT and n = 2 for KO). For d , e , g and j – l , 2 WT clones and 2 KO clones from each line ( n = 4 for each genotype). N = 3 for ( d , e , g , j-l ); N = 1 for a . Scale bars: 300 µm in b; 0.5 µm in c ; 1 µm in h , i .

Journal: Nature Communications

Article Title: A differential requirement for ciliary transition zone proteins in human and mouse neural progenitor fate specification

doi: 10.1038/s41467-025-58554-3

Figure Lengend Snippet: a Log2(tpm+1) data from bulk RNASeq analysis show expression profiles of GLI1 and PTCH1 as mean ± SEM. b , c Immunofluorescence analysis of ciliary proteins in WT and RPGRIP1L-deficient spinal organoids at day 6. b Cilia are labeled by ARL13B and basal bodies by γ-TUBULIN. c Cilia are labeled by IFT81 in green. d , e Cilia length and density measurements in WT and RGPRIP1L -/- organoids at day 6. Data are shown as median with quartiles. Mann-Whitney test was performed for statistical analyzes. f Stimulated-Emission-Depletion (STED) images of WT and RPGRIP1L-deficient cilia at day 6. Cilia are labeled by ARL13B and RPGRIP1L. g Quantification of the ciliary RPGRIP1L amount based on confocal images. Data are shown as median with quartiles. Statistics: unpaired t tests with Welch’s correction ( p < 0.0001). h , i Immunofluorescence of WT and RPGRIP1L-deficient cilia on spinal organoids at day 6. Cilia are labeled by ARL13B and AC3 ( h ) or by ARL13B and INPP5E ( i ). Basal bodies are labeled by γ-TUBULIN. j, k, l Quantifications of ciliary ARL13B ( j ), AC3 ( k ) and INPP5E ( l ) amounts in WT and RPGRIP1L-deficient organoids. Data are shown as median with quartiles. Statistics: unpaired t tests with Welch’s correction ( p < 0.0001). a - l N: number of independent experiments; n: number of different WT or KO clones analyzed per experiment. For a , 2 WT clones and 1 KO clone from each iPSC line ( n = 4 for WT and n = 2 for KO). For d , e , g and j – l , 2 WT clones and 2 KO clones from each line ( n = 4 for each genotype). N = 3 for ( d , e , g , j-l ); N = 1 for a . Scale bars: 300 µm in b; 0.5 µm in c ; 1 µm in h , i .

Article Snippet: STED images were acquired on the STEDYCON confocal and STED module (Abberior Instruments GmbH, Germany) connected to the lateral port of an IX83 inverted microscope (Evident Scientific).

Techniques: Expressing, Immunofluorescence, Labeling, MANN-WHITNEY, Clone Assay